FIG. 2.

Cross-linking of ODV envelope protein cassettes to proteins within microsomal membranes. (a) Schematic of the experimental protocol utilized for chemical cross-linking assays. After in vitro translation and the generation of a radiolabeled protein, the sample was treated with BS³, the microsomal membranes were denatured, and the bait cassette or covalently linked associated complex was enriched using His-binding Talon beads. (b) The enriched samples were separated using SDS-PAGE. O represents the un-cross-linked control, X represents enriched cross-linked samples, and • notes the cross-linked adducts. No detectable cross-linked adduct was detected for Orf91, but the expected site of an adduct comparable to that detected for the other ORFs is noted with a question mark.