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Effect of SOCS1-KIR and Tkip treatment at different times on the replication of vaccinia virus. BSC-40 cells were infected with vaccinia virus at an MOI of 5 for 1 h. Cells were washed and treated with 25 μM lipo-SOCS1-KIR, lipo-Tkip, or the control peptides at time zero and 1 h, 2 h, and 4 h after infection. Cells were allowed to grow for 24 h. Cell extracts and supernatants and were harvested and assayed for the virus in cell extracts (a to d) and supernatants (e to h).
