TABLE 1.
Amino acid sequences of the peptides used in this study and alignment with SOCS5-KIRa
| Peptide | Sequence |
|---|---|
| SOCS1-KIR | 53DTHFRTFRSHSDYRRI |
| SOCS1-KIR2A | 53DTHFATFASHSDYRRI |
| SOCS5-KIR | WKVH-TQIDY |
| Tkip | WLVF-FVIFYFFR |
| Tkip2A | WLVFFVIAYFAR |
| Tkip5A | WLVAAVIAYFAA |
| IFN-γ(95-106) | 95AKFEVNNPQVQR |
| IFN-γ(95-132) | 95AKFEVNNPQVQRQAFNELIRVVHQLLPESSLRKRKRSR |
a
All of the peptides were synthesized with an attached lipophilic group, palmitic acid, for cell penetration. SOCS1-KIR2A and Tkip2A are alanine substitution-containing mutant forms of SOCS1-KIR and Tkip, respectively, that do not show any biological activity. IFN-γ(95-106) is a truncated form of IFN-γ(95-132) and lacks any biological activity. The SOCS5-KIR sequence is presented for comparison with the Tkip sequence. Identical residues in SOCS5-KIR and Tkip, as described by Nicholson and colleagues (11), are in boldface type.