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Purification of recombinant C. trachomatis MurC
| Sample | Concn (mg ml−1) | Total protein (mg) | Purification (fold) | Activity (nmol min−1 mg of protein−1) |
|---|---|---|---|---|
| Soluble fraction | 13.2 | 1,320 | 1 | 5.8 ± 1.0a |
| Nickel-NTA eluate | 0.8 | 17.3 | 76b | 24.8 ± 1.7a |
| Gel filtration eluate | 1.1 | 5.4 | 244b | 73.8 ± 5.1c |
Mean ± standard deviation from two enzymatic determinations
Owing to the presence of chromosomal E. coli MurC in the soluble fraction, this value may be underestimated
Vmax value.
