TABLE 2.

Kinetic parameters of recombinant C. trachomatis MurC

Substratea	Km (mM) (mean ± SD)	Vmax (nmol/min−1 mg of protein−1)	Vmax/Km (nmol min−1 mM−1 mg of protein−1)
UDP-MurNAcb	0.196 ± 0.065	—c	376
ATPb	0.162 ± 0.040	—c	456
l-Alanine	0.124 ± 0.037	73.8 ± 5.1	595
l-Serine	0.242 ± 0.067	76.6 ± 4.4	316
Glycine	1.17 ± 0.19	206 ± 10	176

^aConcentrations of the fixed substrates: ATP, 2.5 mM; UDP-MurNAc, 2 mM; l-alanine, 10 mM. The concentration range of the varied substrates was 0 to 5 mM, except that for glycine, which was 0 to 10 mM.

^bDetermined with l-alanine as the amino acid substrate.

^cThe V_max is the same as that for l-alanine.