TABLE 1.
Survey of induction of pspA by chromosomal mutations in secretion loci of E. coli
| Straina | β-Galactosidase assay result (Miller units)b | Reference |
|---|---|---|
| JS7131(pMR25) ara | 6 ± 0 | 29 |
| JS7131(pSJ1) ara | 59 ± 0.4 | 29 |
| JS7131(pMR25) glu | 8 ± 0.29 | 29 |
| JS7131(pSJ1) glu | 277 ± 1.9 | 29 |
| MC4100(pMR25) | 15.4 ± 0.7 | 8 |
| MC4100(pSJ1) | 183 ± 3.5 | 8 |
| BILKO (MC4100 ΔtatC)(pMR25) | 17 ± 0.3 | 6 |
| BILKO (MC4100 ΔtatC)(pSJ1) | 180 ± 0.17 | 6 |
| ELV15 (MC4100 ΔtatA)(pMR25) | 33 ± 0.9 | 30 |
| ELV15 (MC4100 ΔtatA)(pSJ1) | 176 ± 2.6 | 30 |
| J1M1 (MC4100 ΔtatE)(pMR25) | 31 ± 0.8 | 30 |
| J1M1 (MC4100 ΔtatE)(pSJ1) | 160 ± 0.9 | 30 |
| JARV15 (MC4100 ΔtatA ΔtatE)(pMR25) | 34 ± 0.4 | 30 |
| JARV15 (MC4100 ΔtatA ΔtatE)(pSJ1) | 172 ± 1.7 | 30 |
| HPT57 (MC1000 phoA+ phoR leu+ 102::malF-lacZ102)(pMR25) | 3.6 ± 0.7 | 33 |
| HPT57 (MC1000 phoA+ phoR leu+ 102::malF-lacZ102)(pSJ1) | 39 ± 0.8 | 33 |
| HPT-UV46 (ffs-46) (pMR25) | 4.6 ± 0.8 | 33 |
| HPT-UV46 (ffs-46) (pSJ1) | 45.6 ± 2.9 | 33 |
| HPT-UV70 (ftsY70) (pMR25) | 5 ± 2.3 | 33 |
| HPT-UV70 (ftsY70) (pSJ1) | 26 ± 0.8 | 33 |
| HPT-UV73 (secM73) (pMR25) | 5.2 ± 0.2 | 33 |
| HPT-UV73 (secM73) (pSJ1) | 418 ± 0.8 | 33 |
| HPT-UV77 (ffh-77) (pMR25) | 2.2 ± 1.3 | 33 |
| HPT-UV77 (ffh-77) (pSJ1) | 34 ± 0.65 | 33 |
| HPT-UV87 (ffh-87) (pMR25) | 4.8 ± 0.3 | 33 |
| HPT-UV87 (ffh-87) (pSJ1) | 23.1 ± 0.84 | 33 |
| HPT-UV89 (ffh-89) (pMR25) | 2.9 ± 0.9 | 33 |
| HPT-UV89 (ffh-89) (pSJ1) | 49 ± 2.2 | 33 |
| HPT-UV93 (secM93) (pMR25) | 7.4 ± 2.3 | 33 |
| HPT-UV93 (secM93) (pSJ1) | 420 ± 9.9 | 33 |
| HPT-UV103 (ffh-103) (pMR25) | 4.9 ± 0.49 | 33 |
| HPT-UV103 (ffh-103) (pSJ1) | 37.7 ± 0.27 | 33 |
| MC4100(pMR25)c | 1.36 ± 0.79 | 4 |
| MC4100(pMR25)d | 2.57 ± 0.8 | 4 |
| MC4100(pSJ1)c | 37.3 ± 0.8 | 4 |
| MC4100(pSJ1)d | 38.4 ± 0.6 | 4 |
| HDB45(pMR25)c | 3.0 ± 0.58 | 4 |
| HDB45(pMR25)d | 3.3 ± 0.86 | 4 |
| HDB45(pSJ1)c | 37.1 ± 0.35 | 4 |
| HDB45(pSJ1)d | 36.4 ± 1.09 | 4 |
Where present, the pMR25 vector numbered one to five copies per cell (C. Mohr and R. Roberts, unpublished data) (a gift from Dickon Alley). ara or glu, cultures were grown with LB plus 0.2% arabinose or glucose, respectively. pSJ1 is pMR25 with a pspA-lacZ transcriptional fusion. JS7131 strains were supplied by T. Palmer. The BILKO, ELV15, J1M1, and JARV15 strains, as well as the MC4100(pMR25) and MC4100(pSJ1) strains described in reference 8, were supplied by R. Dalbey. The HPT strains were supplied by J. Beckwith. The HBD strains and the MC4100(pMR25) and MC4100(pSJ1) strains described in reference 4 were supplied by Harris Bernstein.
Assays were in triplicate using bacteria grown in LB supplemented with the appropriate antibiotic (tetracycline at 10 μg/ml). Results are expressed as means ± standard errors of the means.
Grown with IPTG.
Grown without IPTG.