TABLE 3.
Comparison of experimental pseudorevertant restriction fragment sizes that complemented the T192H mutant to photoautotrophic growth, with the theoretical restriction pattern of the sole region of the Synechocystis sp. strain PCC 6803 genome that fits these criteria
| Enzyme | DNA fragment size in fraction that complements photoautotrophic growth (kb) | Actual size of the fragment (bp) | Position of DNA fragment that includes secondary mutation sitea |
|---|---|---|---|
| BamHI | —b | 1,831 | 754522-756352 |
| BspEI | 12.0-20.0 | 17,772 | 745500-763271 |
| EcoRV | 12.0-20.0 | 19,133 | 737521-756653 |
| KpnI | 10.0-15.0 | 12,143 | 747099-759241 |
| PstI | 10.0-15.0 | 14,878 | 740038-754915 |
| SmaI | 12.0-17.0 | 16,525 | 752366-768890 |
a
Numbering according to CyanoBase.
b
—, this fragment did not complement T192H to photoautotrophic growth, because one of the restriction sites for this enzyme, 754521 is close to the secondary mutation site, 754308.