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Examination of periplasmic TolC intermediates from strains expressing wild-type TolC (TolCwt), TolCI106N, and TolCS350F. TolC and MBP were immunoprecipitated from purified periplasmic fractions obtained from pulse-chase experiments. (A) Autoradiographs of SDS-polyacrylamide gels carrying immunoprecipitated samples. (B) TolC levels, relative to MBP levels, were quantified and graphed. ♦, TolCwt; ▪, TolCI106N; ▴, TolCS350F.
