TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Characteristics | Source or reference |
|---|---|---|
| Strains | ||
| MC4100 | F−araD139 Δ(argF-lac)U139 rpsL150 flbB5301 ptsF25 deoC1 thi-1 rbsR relA | 7 |
| RAM958 | MC4100 tolC::Tn10-48 | 42 |
| RAM1149 | RAM958/pTrc99A | This study |
| RAM1150 | RAM958/pTrc-tolC+ (BspHI-HindIII clone) | This study |
| RAM1151 | RAM958/pTrc-tolC(TolC1106N) | This study |
| RAM1152 | RAM958/pTrc-tolC(TolCS350F) | This study |
| RAM787 | MC4100 rcsB::Kmr | 22 |
| RAM789 | MC4100 rcsB::Kmr Δrfa-2057 (Cmr) | 22 |
| RAM913 | MC4100 ΦdegP::lacZ+ Tn10::zad-475 | R. Misra |
| RAM914 | MC4100 ΦdegP::lacZ+ Δskp Tn10::zad-475 | R. Misra |
| RAM951 | MC4100 surA::Kmr | R. Misra |
| RAM1116 | RAM958 surA::Kmr/pTrc-tolC+ | This study |
| RAM1117 | RAM958 Δrfa-2057/pTrc-tolC+ | This study |
| XL1-Red | endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac mutD5 mutS mutT::Tn10 | Stratagene |
| Plasmid | ||
| pTrc99A | Apr; expression vector | Pharmacia |