TABLE 2.
Primers used
| Primer | Sequencea |
|---|---|
| LFhns-S | 5′-caaaataaGtcgaCttgcttaaaggc-3′ (SalI site) |
| RRhns-X | 5′-cgttattctAgacgaatatgagtccga-3′ (XbaI site) |
| Mar3-2 | 5′-ccggggacttatcagccaacctgttat-3′ |
| S1B-CAT5 | 5′-ggtcgacggatccggggaat-3′ |
| S1S-CAT3 | 5′-caagcaggtcgacatcttttctgttcat-3′ |
| ompS1-PE | 5′-ttgctgcgcctgccactaataac-3′ |
| ompA-PE | 5′-tttgcgcctcgttatcatccaa-3′ |
| 310b-1 | 5′-tagccttttatcatttattttatc-3′ |
| 310b-2 | 5′-atgagttatgtgttttgatttga-3′ |
| 310b-3 | 5′-caaagcatcaaatacatataaaaa-3′ |
| 310b-4 | 5′-tgtttctatttggttttttataatac-3′ |
| 310b-7 | 5′-attcttagtcacttatatatcctgtattat-3′ |
| 310b-8 | 5′-aatatgtagccacttcaacaaaac-3′ |
a
Uppercase letter indicate changes in the primer sequence with respect to the wild type, designed to introduce restriction enzyme sites.