TABLE 2.
Levels of MSH and precursors in M. tuberculosis mshC disruption clones
| Strain | Optical density (600 nm) | Cellular content (nmol/109 cells)a
|
||
|---|---|---|---|---|
| GlcNAc-Ins | GlcN-Ins | MSH | ||
| Wild-type M. tuberculosis Erdman | 0.50 | 1.7 ± 0.7 | 8.9 ± 0.2 | 13.7 ± 0.2 |
| Mtb1682 (2X-mshC) | 0.85 | 0.9 ± 0.1 | 3.2 ± 0.1 | 26.2 ± 1.2 |
| Mtb1682 transformant | ||||
| Clone 3 | 0.41 | ≤0.71 | 7.3 ± 0.1 | 12.1 ± 1.3 |
| Clone 14 | 0.36 | ≤1.4 | 14.1 ± 0.2 | 12 ± 0.3 |
| Clone 16 | 0.37 | ≤0.6 | 5.7 ± 0.3 | 14.6 ± 0.1 |
| Clone 18 | 0.43 | ≤1.2 | 12 ± 1 | 10.2 ± 0.1 |
| Clone 157 | 0.58 | 1.5 ± 0.7 | 12 ± 1.4 | 10.2 ± 0.1 |
| Clone 158 | 0.52 | 1.7 ± 0.4 | 15 ± 1 | 11.8 ± 0.1 |
| Clone 172 | 0.49 | 1.0 ± 0.5 | 8.5 ± 0.2 | 11.3 ± 0.2 |
a
Results are expressed as the means and ranges of duplicate samples.