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. 2003 Nov;185(22):6562–6574. doi: 10.1128/JB.185.22.6562-6574.2003

FIG. 3.

FIG. 3.

RT-PCR analysis of transcription of yeiG, pyrG, and orf in L. lactis. (A) Physical map of the pyrG region from L. lactis. Numbering refers to the DNA sequence submitted to the EMBL data library and assigned accession number AJ010153. The theoretical PCR products are shown. a, PCR fragment obtainable with oligonucleotides SLLH17 and SLLH18; b, PCR fragment obtainable with oligonucleotides SLLH19 and pyrG9b; c, PCR fragment obtainable with oligonucleotides SLLH17 and pyrG9b; d, PCR fragment obtainable with oligonucleotides pyrG8a and SLLH7. (B) Agarose gel electrophoresis of the RT-PCR products. Lanes PCR/DNA, ordinary PCR with DNA as the template; lanes RT-PCR, RT-PCR with RNA as the template; PCR/RNA, PCR with RNA as the template. a, b, c, and d refer to the theoretical PCR fragments shown in panel A. Lanes M, 1-kb Plus DNA ladder from GibcoBRL.