FIG. 8.

Predicted structures of terminators and antiterminators in the 5′ pyrG leader sequences from L. lactis (accession number AJ010153) B. subtilis (accession number Z49782), and E. faecalis (accession number AE016950). The first nucleotide in the mRNA is marked +1. The +1 positions in E. faecalis and L. lactis are not supported by experimental evidence, whereas the +1 position of B. subtilis was determined by Meng and Switzer (25). The translational start codons are found further downstream. The stems in the terminator structure are marked by boxes in both the terminator and the antiterminator. +45 and +69 in the structure for L. lactis indicate the deletion points in plasmids pCJ35 and pCJ36 (Fig. 7).