FIG. 2.

Architecture of crgA and surrounding genes. The S. avermitilis crgA gene (SAV4331) is represented by the solid arrow. The position of insertion of Tn1792 in the crgA mutant is indicated by the triangle. The adjacent sequenced open reading frames are indicated by hatched arrows. The positions of the SalI sites used to clone out the tagged copy of crgA are indicated (S). The sequence beyond the left-hand SalI site was not determined, and the extent of the divergently transcribed gene is an estimate based on the corresponding orthologous gene in the S. coelicolor genome. The positions of the sequences complementary to the PCR primers used to obtain the full-length crgA gene for complementation (M219SCS and M219SCA) are shown.