FIG. 3.
crgA influences reproductive growth in Streptomyces. (A) Surface view of cultures grown on MS agar for 7 days. a, S. avermitilis 12804 (wild type); b, S. avermitilis DM219; c, S. avermitilis DM219(pSET152); d to h, S. avermitilis DM219(pSET219). (B) Cultures were grown on NE medium for 3 days. a, S. coelicolor M145; b, S. coelicolor DC3854; c, S. coelicolor DC3854/pSC3854; d, S. coelicolor DC3854/pSET152. (C) Cultures were grown on MS medium containing 2.5 μg of thiostrepton ml−1 for 5 days. a, S. coelicolor M145; b, S. coelicolor M145/pIJ8600; c, S. coelicolor DC3854; d, S. coelicolor M145/pME41.