Fig.4. Migration of HEK 293 cells expressing a high density of α_Dβ₂ to various ECM proteins.

HEK 293 cells expressing a high density of α_Dβ₂ were analyzed for their ability to migrate to 50 µg/ml fibrinogen, fibronectin and CCN1 in the absence or in the presence of blocking polyclonal anti-α_D antibody or blocking anti-β₁ mAb. Cells were preincubated with 20 µg/ml antibodies for 20 min before their addition to the upper chamber of Transwell plates. Cells were allowed to migrate toward ligands for 18 hours at 37°C in 5% CO₂ and the extent of cell migration was assessed as described in Materials and Methods. Data are expressed as a percentage of migration; cell migration in the absence of blocking antibodies was assigned a value of 100%. Values represent the means ± SD with duplicate wells for each experiment from three or more individual experiments (*, P<0.05; **, P<0.01 compared to control).