Fig.5. Analyses of integrin αDβ2 and αMβ2 expression on mouse IC-21 macrophages after PMA stimulation.
IC-21 cells were stimulated with 100ng/ml PMA for different periods of time and the levels of αMβ2 (open bars) and αDβ2 (black bars) integrins were assessed by flow cytometry using anti-αM mAb M1/70 and polyclonal anti-αD antibody. Results are the mean ± SD values of mean fluorescence from three independent experiments (*, P<0.05; **, P<0.01 compared to αD expression before PMA treatment.)