Fig.8. Effect of anti-α_D antibodies on macrophage accumulation in the peritoneum.

Mice were treated with affinity purified anti-α_D polyclonal antibody (100 µg) or rabbit IgG 4 hours before the development of peritonitis. Peritonitis was induced by intraperitoneal injection of 3% Brewer thioglycollate medium (0.5 ml). 72 hours later, the mice were euthanized by isoflurane inhalation, and their peritoneal cavities were lavaged with 5 ml PBS. The numbers of migrated macrophages in the peritoneum were determined by nonspecific esterase assay. The data shown are representative of four separate experiments. The data represent the means ± SD for the control and the treated groups with seven mice in each group. Asterisk denotes the statistically significant difference between the control and treated groups (P < 0.02). This experiment was replicated 4 times with the same results.