TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Insert (description) | Source or reference |
|---|---|---|
| E. coli strains | ||
| TOP10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacZ74 deoR recAI araD139 Δ(ara-leu)7697 galU galK rpsL endAI rupG | Invitrogen |
| MG1655 | F− λ−ilvG rfb-50 rph-1 | 7 |
| Plasmids and constructs | ||
| pBAD18 | None | 20 |
| pRD020 | mazF | This study |
| pRD022 | pspC | This study |
| pRD023 | ykfI | This study |
| pRD024 | yeeV | This study |
| pRD025 | ydjM | This study |
| pRD026 | yafO | This study |
| pRD027 | yfjG | This study |
| pRD028 | ydgF | This study |
| pRD029 | yafW | This study |
| pRD030 | yeeU | This study |
| pRD041 | ykfI-His6 | This study |
| pRD042 | yeeV-His6 | This study |
| pRD049 | yafW-ykfI | This study |
| pRD050 | yeeU-yeeV | This study |
| pRD052 | ypjF | This study |
| pRD061 | yafW-ykfI-His6 | This study |
| pRD062 | yeeU-yeeV-His6 | This study |
| pRD068 | ykfH | This study |
| pRD069 | yeeT | This study |
| pRD086 | yfjZ | This study |
| pRD095 | yeeU-BamHI-yeeV | This study |
| pRD103 | ypjJ | This study |
| pRD104 | yeeT-yeeV | This study |
| pRD105 | gfp-yeeV | This study |
| pRD106 | ykfH-ykfI | This study |
| pRD107 | gfp-ykfI | This study |
| pRD114 | relE | This study |
| pRD124 | gfp-ykfI-His6 | This study |
| pRD125 | ypjF-His6 | This study |
| pRD134 | UTR-yeeV | This study |
| pRD135 | yeeU-UTR-BamHI-yeeV | This study |
| pRD136 | Flag-ypjF | This study |
| pRD139 | yafW-BamHI-ykfI | This study |
| pRD140 | gfp-yeeV-His6 | This study |
| pRD141 | yeeU-BamHI-ykfI | This study |
| pRD142 | yafW BamHI-yeeV | This study |
| pBAD33 | None | 20 |
| pRD072a | yeeV | This study |
a
Low-copy-number pBAD33-based construct. All others described are high-copy pBAD18-based constructs.