TABLE 3.
Effect of coexpression of the adjacent gene, the distal gene, or gfp on the growth inhibition induced by yeeV and ykfI
Plasmid (gene[s] expressed) | Arabinose | Growth at 6 ha |
---|---|---|
pRD069 (yeeT) | + | +++ |
pRD030 (yeeU) | + | +++ |
pRD024 (yeeV) | + | − |
pRD024 (yeeV) | − | +++ |
pRD104 (yeeT-yeeV) | + | − |
pRD050 (yeeU-yeeV) | + | +++ |
pRD105 (gfp-yeeV) | + | − |
pRD068 (ykfH) | + | +++ |
pRD029 (yafW) | + | +++ |
pRD023 (ykfI) | + | − |
pRD023 (ykfI) | − | +++ |
pRD106 (ykfH-ykfI) | + | − |
pRD049 (yafW-ykfI) | + | +++ |
pRD107 (gfp-ykfI) | + | − |
pRD103 (ypjJ) | + | +++ |
pRD086 (yfjZ) | + | +++ |
pRD052 (ypjF) | + | +++ |
Growth curves were performed as described for Fig. 2. Optical density after 6 h of growth is represented as follows: −, OD600 < 0.5; +++, OD600 > 1.5.