TABLE 5.
The unique 68-bp UTR between yeeU and yeeV is required for yeeU antitoxin function
| Plasmid (gene[s] expressed) | Arabinose | Growth at 6 ha |
|---|---|---|
| pRD024 (yeeV) | + | − |
| pRD024 (yeeV) | − | +++ |
| pRD095 (yeeU-BamHI-yeeV) | + | − |
| pRD134 (UTR-yeeV) | + | + |
| pRD135 (yeeU-UTR-BamHI-yeeV) | + | +++ |
| pRD050 (yeeU-yeeV) | + | +++ |
| pRD142 (yafW BamHI-yeeV) | + | +++ |
| pRD072 (yeeV) | + | − |
| pRD072 (yeeV) + pRD030 (yeeU) | + | − |
| pRD023 (ykfI) | + | − |
| pRD023 (ykfI) | − | +++ |
| pRD139 (yafW-BamHI-ykfI) | + | +++ |
| pRD049 (yafW-ykfI) | + | +++ |
| pRD141 (yeeU-BamHI-ykfI) | + | ++ |
a
Growth curves were performed as described for Fig. 2. Optical density after 6 h of growth is represented as follows: −, OD600 < 0.5; +, 0.5 < OD600 < 1.0; ++, 1.0 < OD600 < 1.5; +++, OD600 > 1.5.