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Phase-contrast microscopy of B. subtilis 168 (wild type) (a and b), EFD (lytE) (c and d), E3FLFd (lytE lytF-3xFLAG) (e and f), ED (lytF) (g and h), and F3FLEd (lytF lytE-3xFLAG) (i and j). All strains were cultured in LB medium at 30°C. Cells were harvested and fixed as described in Materials and Methods at the early exponential phase (OD600, 0.5) (a, c, e, g, and i) and the mid-exponential phase (OD600, 1.5) (b, d, f, h, and j). Bars = 10 μm.
