FIG. 1.

RPA to analyze the mRNA levels of sp1803 and sp1804 in wild-type strain AC353 (lanes wt), mgrA deletion strain AC1500 (lanes Δ), and mgrA-overexpressing strain AC1481 (lanes +). Riboprobes for sp1803, sp1804, and rpoB were generated and hybridized to 10 μg of S. pneumoniae RNA from the three strains. RNA was harvested from cells grown in 0.8% maltose to an OD₆₀₀ of 0.4. Lanes U and D contained undigested riboprobes and riboprobes digested by RNase in the absence of S. pneumoniae RNA, respectively.