FIG. 4.

RPAs to analyze the mRNA levels of a gene in each transcriptional unit in the rlrA islet in wild-type strain AC353 (lanes wt), mgrA deletion strain AC1500 (lanes Δ), and mgrA-overexpressing strain AC1481 (lanes +). Riboprobes for each gene, as well as rpoB, were generated and hybridized to 10 μg of S. pneumoniae RNA from the three strains. RNA was harvested from cells grown in 0.8% maltose to an OD₆₀₀ 0.4 or 0.6. Transcript levels were comparable at either time point. The results obtained with an OD₆₀₀ of 0.4 are shown. Lanes U and D contained undigested riboprobes and riboprobes digested by RNase in the absence of S. pneumoniae RNA, respectively.