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RPA to analyze the mgrA mRNA levels in wild-type strain AC353 (lane wt), rlrA deletion strain AC1213 (lane Δ), and rlrA-overexpressing strain AC1278 (lane +). Riboprobes for mgrA and rpoB were generated and hybridized to 10 μg of S. pneumoniae RNA from the three strains. RNA was harvested from cells grown in 0.8% maltose to an OD600 of 0.4. Lanes U and D contained undigested riboprobes and riboprobes digested by RNase in the absence of S. pneumoniae RNA, respectively.
