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. 2003 Nov;185(22):6719–6722. doi: 10.1128/JB.185.22.6719-6722.2003

FIG. 2.

FIG. 2.

Effect of the secM membrane anchor sequence on secA regulation. CG155 (MC1000 recA) and CG29 [MC1000 secD1(Cs) phoR recA1 srl::Tn10] containing pPhIF or pPhIF-MA were grown in Luria broth containing 100 μg of ampicillin per ml at 39°C to mid-logarithmic phase when the cultures were shifted to 23°C for 4 h. β-Galactosidase assays were performed in duplicate for each of two duplicate cultures as described previously (11). The average result is given with the error bar indicating the standard deviation.