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Glycosylation of flagellins from the wild type (WT) and the Δorf1, Δorf2, and Δorf3 mutants of P. syringae pv. glycinea. (A) Western blot analysis of the flagellin protein in the total proteins. (B) Coomassie brilliant blue (CBB) staining of the purified flagellins. (C) Staining of the glycosyl moiety in purified flagellin.
