TABLE 1.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Relevant characteristicsa | Reference or source |
|---|---|---|
| E. coli strains | ||
| DH5a | F−λ− φ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK+) supE44 thi-1 gyrA relA1 | Takara, Kyoto, Japan |
| S17-1 | thi pro hsdR hsdM+recA [chr::RP4-2-Tc::Mu-Km::Tn7] | 22 |
| P. syringae pv. glycinea strains | ||
| race 4 | Wild type, Nalr | A. Collmer |
| race 4-d1 | race 4 Δorf1 | This study |
| race 4-d2 | race 4 Δorf2 | This study |
| race 4-d3 | race 4 Δorf3 | This study |
| Plasmids | ||
| pUC18 | 2.7-kb cloning vector, Apr | Takara, Kyoto, Japan |
| pUCSac1 | 1.8-kb SacI-SacI fragment in pUC18, Apr | This study |
| pUCSac2 | 13.4-kb SacI-SacI fragment in pUC18, Apr | This study |
| pUCSal1 | 1.5-kb SalI-SalI fragment in pUC18, Apr | This study |
| pCR-blunt II-TOPO | 3.5-kb cloning vector for PCR blunt end product, Kmr | Invitorgen |
| pK18mobsacB | Small mobilizable vector, Kmr, sucrose sensitive (sacB) | 22 |
| pM1 | 2.18-kb chimeric PCR product deleting orf1 cloned into pK18mobsacB at EcoRI site, Kmr | This study |
| pM2 | 1.79-kb chimeric PCR product deleting orf2 cloned into pK18mobsacB at EcoRI site, Kmr | This study |
| pM3 | 1.69-kb chimeric PCR product deleting orf3 cloned into pK18mobsacB at EcoRI site, Kmr | This study |
a
Apr, ampicillin resistance; Kmr, kanamycin resistance, Nalr, nalidixic acid resistance.