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Dose-dependent binding of PcrA to duplex DNA substrates containing 5′ or 3′ ss regions (oligonucleotide [oligo] c or oligonucleotide d, respectively). 32P-labeled oligonucleotides were incubated with the indicated amounts of PcrA, and the products were resolved by polyacrylamide gel electrophoresis. P, free probe; C, PcrA-DNA complex.
