TABLE 1.
Effects of rlrA and rorA on the transformation frequency in Streptomyces of circular pSLA2 plasmid constructs
| Expt no. | Plasmid | rlrA | rorA | Transformation frequency in Streptomyces (transformants/μg of DNA)a |
|---|---|---|---|---|
| 1 | pQC36 | − | − | 3 × 104 |
| 6 | pQC91 | + | − | 3 × 101 |
| 7 | pQC125 | + | − | 4 × 101 |
| 8 | pQC61 | − | + | 1 × 104 |
| 9 | pQC48 | + | + | 4 × 104 |
| 12 | pQC101 | + | + | 3 × 104 |
a
A 0.1-μg aliquot of pSLA2 circular plasmid DNA isolated from E. coli was introduced into S. lividans ZX7 by transformation (16). Transformant colonies were counted after thiostrepton selection.