Figure 6.—

Analysis of plasmid loss. To determine if srp1 mutant cells have defects in DNA replication, a plasmid loss assay was performed. Plasmid loss was determined in cells containing plasmids with early (ARS305)-, middle (ARS1)-, or late (ARS1412)-firing ARSs as described in materials and methods. Results are plotted as the mean percentage of plasmid loss for (A) ARS305 (early)-, (B) ARS1 (middle)-, or (C) ARS1412 (late)-firing ARS sequences for each srp1 mutant (srp1-31, srp-55, and srp1-E402Q) as well as for wild-type (SRP1) control cells. Experiments were performed in triplicate. Standard deviations in the data are indicated by the error bars. A one-way ANOVA followed by a Dunnett's multiple comparison test using an α-value of 0.05 was used to determine the significance of the results. Significant differences between samples corresponding to the P-values of *P < 0.05 and **P < 0.01 are indicated by the lines above the bars.