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Cost of synthesis per building block
| Cost ($) | |
|---|---|
| Templateless PCR | |
| Oligos (18 70 mers) | 113.40 |
| Reagents | 0.14 |
| Plastics | 0.57 |
| Subtotal | 114.11 |
| Finish PCR: reagents and plastics | 0.97 |
| Ligation and transformation | |
| Ligation kit (Promega pGEM-T) | 2.75 |
| Competent cells (Promega JM109) | 2.75 |
| LB + carbenicillin plate | 0.47 |
| Plastics | 0.18 |
| Subtotal | 6.15 |
| Colony screening PCR | |
| Plastics | 1.17 |
| Reagents | 2.58 |
| Subtotal | 3.75 |
| Sequencing | |
| Plastics | 1.44 |
| Sequencing (18 clones) | 90.00 |
| Subtotal | 91.44 |
| Total | 216.42 |
