Figure 1.—

Ubiquitous silencing of EcR-B1 in the follicular epithelium induces egg chamber degeneration. Confocal cross-sections of ovarioles from females overexpressing the UAS-IR-EcR-B1 transgene under the control of the tub-Gal4 promoter (A–F) and from wild-type females (G). FITC-phalloidin (A and D; green), propidium iodide staining (B and E; red), and their merged signals (C and F) show the presence of degenerated egg chambers inside ovarioles (see white arrows). In comparison, in the wild-type ovariole (G) FITC-phalloidin and propidium iodide merged signals illustrate the absence of degenerating egg chambers. Anterior is up in all panels. Germ, germarium; st, stage. Bars, 20 μm.