TABLE 5.
Substitutions in 43° adapted lines
| Ancestral residues |
|||||||
|---|---|---|---|---|---|---|---|
| Species | Substitution | Gene region | Amino acid change | MS2 | fr | NL95 | No. lines |
| MS2 | C206U | M | Thr 26 Ile | Leu 26 | Val 10 | 3/3 | |
| U466C | M | Tyr 113 His | Trp 113 | Leu 111 | 3/3 | ||
| C1549A/U | C | Thr 72 Ile/Asn | Val 72 | Thr 72 | 2/3 | ||
| G1551A | C | Val 73 Ile | Gln 73 | Ala 73 | 1/3 | ||
| U1685C | C | a | — | — | 2/3 | ||
| L | Ile 3 Thr | b | — | ||||
| U2733G | R | Ser 325 Ala | Ala 325 | Ser 342 | 1/3 | ||
| C3224U | R | a | — | — | 1/3 | ||
| C3320U | R | a | — | — | 2/3 | ||
| fr | U908C | M | a | — | — | 1/1 | |
| A1140G | M | Ile 338 Val | Pro 338 | Leu 393 | 1/1 | ||
| C1697A | C | Thr 121 Lys | Ser 121 | Asp 123 | 1/1 | ||
| L | Gln 3 Lys | Gln 8 | — | ||||
| A1756U | L | Glu 22 Asp | Asp 26 | — | 1/1 | ||
| U1785C | R | a | — | — | 1/1 | ||
| L | Ser 32 Lys | Ser 36 | — | ||||
| U2271C | R | a | — | — | 1/1 | ||
| G3421A | 3′UTR | — | — | — | 1/1 | ||
| NL95 | U275C | M | Phe 75 Leu | Gly 73 | Gly 73 | 3/3 | |
| G491A | M | Ala 147 Thr | Val 141 | Val 141 | 2/3 | ||
| G904A | M | a | — | — | 3/3 | ||
| U1216G | M | a | — | — | 1/3 | ||
| A1447G | C(T) | Asn 17 Asp | Asp 18 | Asp 18 | 1/3 | ||
| G1618A | C(T) | Ala 74 Thr | Val 73 | Gln 73 | 3/3 | ||
| G2075A | T | Gly 226 Glu | — | — | 3/3 | ||
| U2529C | R | Val 40 Ala | b | b | 2/3 | ||
| U3376C | R | a | — | — | 2/3 | ||
| C3535U | R | a | — | — | 1/3 | ||
Gene regions are maturase (M), major coat (C), lysis (L), read-through (T), replicase (R), and 3′-untranscribed region (3′-UTR). The coat and read-through coding regions in NL95 are in frame and therefore share any changes affecting amino acid residues. The effects of nonsynonymous changes are shown as the ancestral residue–residue position–derived residue (e.g., Thr 26 Val). —, either the change was synonymous or the coding region is absent.
a
A synonymous change.
b
Residues for which no homologous amino acid exists in a related species.