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Effect of terminator and repressor sequences upstream of MRA cassette on frequency of small MRA escape colonies. Overnight grown cultures of (A) CBY5565b (no terminator), (B) Y15110-3-4.1b (no terminator), (C) Y15111b (TTEF1) and (D) Y15114b (PGAL1-TADH1) were normalized to same optical density, and 500 μl was plated on SC−His media. Plates were imaged after 3.5 days.
