Figure 3.—

Escape from α-regulated MRA is reduced by deletion of HMLα and not associated with sterile MRA escape colonies. MRA escape and mating type of MRA escape colonies was measured for (A) Y15577-2.1a (can1Δ0∷P_MFα1-his5⁺/HMLα) and (B) Y15579-4a (can1Δ0∷P_MFα1-his5⁺/hmlΔ0∷URA3ca) as described in Figure 2. Note that MATa colonies (highlighted by circles) tend to be smaller, all colonies mate (i.e., no sterile colonies), and mating-type switching is eliminated by deletion of HMLα.