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A high frequency of MRA escape observed in heterozygous diploids obtained by mating query and deletion array strains is eradicated by canavanine, but not thialysine. Diploid clones, heterozygous for lyp1Δ0/LYP1 and can1Δ0/CAN1 alleles were formed by mating hoΔ0∷KanMX strains (from Research Genetics, Huntsville, AL) with (A) Y15578-1.2b or (B) Y15588-10.2a. MRA escape was assayed on −HIS media, and −HIS media +thialysine or canavanine, as described in Figure 2.
