TABLE 1.
List of yeast strains used in this study
| Strain name | Relevant genotype | Acquired from |
|---|---|---|
| i. | ||
| CBY5565b | MATα his3Δ1 leu2Δ0 ura3Δ0 met17Δ0 lyp1Δ0 mfα1Δ∷PMFα1-LEU2 can1Δ0∷PMFA1-HIS3 | Boone laboratory |
| CBY7092b | MATα his3Δ1 leu2Δ0 ura3Δ0 met17Δ0 lyp1Δ0 can1Δ0∷PSTE2-his5+ | Burke laboratory |
| BY4741a | MATahis3Δ1 leu2Δ0 ura3Δ0 met17Δ0 | Research Genetics |
| BY4742b | MATα his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 | Research Genetics |
| Y15110-3-4.1b | MATα his3Δ1 leu2Δ0 ura3Δ0 met17Δ0 can1Δ0∷PMFA1-HIS3 | This study |
| Y15111b | MATα his3Δ1 leu2Δ0 ura3Δ0 met17Δ0 can1Δ0∷KanMX-TTEF1-PMFA1-HIS3 | This study |
| Y15114b | MATα his3Δ1 leu2Δ0 ura3Δ0 met17Δ0 can1Δ0∷KanMX-PGAL1-TADH1-PMFA1-HIS3 | This study |
| Y15116a | MATahis3Δ1 leu2Δ0 ura3Δ0 met17Δ0 can1Δ0∷PGAL1-TADH1-PMFA1-HIS3 | This study |
| Y15117b | MATα his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 can1Δ0∷PGAL1-TADH1-PMFA1-HIS3 | This study |
| ii. | ||
| Y15118a | MATahis3Δ1 leu2Δ0 ura3Δ0 met17Δ0 can1Δ0∷PGAL1-TADH1-PMFA1-LEU | This study |
| Y15125-1.3b | MATα his3Δ1 leu2Δ0 ura3Δ0 can1Δ0∷PGAL1-TADH1-PMFA1-LEU2 | This study |
| Y15121a | MATahis3Δ1 leu2Δ0 ura3Δ0 met17Δ0 lys2Δ0∷PGAL1-TADH1-PMFA1-HIS3 | This study |
| Y15122a | MATahis3Δ1 leu2Δ0 ura3Δ0 met17Δ0 lys2Δ0∷PGAL1-TADH1-PMFA1-LEU2 | This study |
| Y15126-1.4a | MATahis3Δ1 leu2Δ0 ura3Δ0 met17Δ0 lys2Δ0∷PGAL1-TADH1-PMFA1-HIS3 | This study |
| Y15124-2.4b | MATα his3Δ1 leu2Δ0 ura3Δ0 met17Δ0 can1Δ0∷PGAL1-TADH1-PMFA1-HIS3 | This study |
| Y15132-2-2.1b | MATα his3Δ1 leu2Δ0 ura3Δ0 can1Δ0∷PGAL1-TADH1-PMFA1-LEU2 | This study |
| Y15132-2-2.4b | MATα his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0∷PGAL1-TADH1-PMFA1-HIS3 | This study |
| Y15132-2-2.3a | MATahis3Δ1 leu2Δ0 ura3Δ0 met17Δ0 can1Δ0∷PGAL1-TADH1-PMFA1-LEU2 | This study |
| Y15127-2.2a | MATahis3Δ1 leu2Δ0 ura3Δ0 lys2Δ0∷PGAL1-TADH1-PMFA1-LEU2 | This study |
| Y15131-1-5.1b | MATα his3Δ1 leu2Δ0 ura3Δ0 can1Δ0∷PGAL1-TADH1-PMFA1-HIS3 | This study |
| Y15131-1-6.3b | MATα his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0∷PGAL1-TADH1-PMFA1-LEU2 | This study |
| iii. | ||
| Y15571a | MATahis3Δ1 leu2Δ0 ura3Δ0 met17Δ0 can1Δ0∷PGAL1-TADH1-PMFA1-his5+ | This study |
| Y15147b | MATα his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 hmrΔ0∷URA3ca | This study |
| Y15572-2-10.3b | MATα his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 can1Δ0∷PGAL1-TADH1-PMFA1-his5+ | This study |
| Y15572-3-30.3b | MATα his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 can1Δ0∷PGAL1-TADH1-PMFA1-his5+ hmrΔ0∷URA3ca | This study |
| iv. | ||
| Y15573b | MATα his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 can1Δ0∷PGAL1-TADH1-PMFα1-his5+ | This study |
| Y15574-3.4a | MATahis3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 can1Δ0∷PGAL1-TADH1-PMFα1-his5+ | This study |
| v. | ||
| CBY5257b | MATα his3Δ1 leu2Δ0 ura3Δ0 met17Δ0 lyp1Δ0 can1Δ0∷PMFA1-HIS3-PMFα1-LEU2 | Boone laboratory |
| Y15575-6.2b | MATα his3Δ1 leu2Δ0 ura3Δ0 met17Δ0 lyp1Δ0 | This study |
| Y15575-2.1a | MATahis3Δ1 leu2Δ0 ura3Δ0 met17Δ0 lyp1Δ0 | This study |
| Y15576-15.3a | MATahis3Δ1 leu2Δ0 ura3Δ0 can1Δ0∷PGAL1-TADH1-PMFA1-his5+ lyp1Δ0 | This study |
| Y15576-12.4b | MATα his3Δ1 leu2Δ0 ura3Δ0 met17Δ0 can1Δ0∷PGAL1-TADH1-PMFA1-his5+ lyp1Δ0 hmrΔ0∷URA3ca | This study |
| Y15578-1.2b | MATα his3Δ1 leu2Δ0 ura3Δ0 can1Δ0∷PGAL1-TADH1-PMFA1-his5+ lyp1Δ0 hmrΔ0∷URA3ca | This study |
| Y15577-2.1a | MATahis3Δ1 leu2Δ0 ura3Δ0 can1Δ0∷PGAL1-TADH1-PMFα1-his5+ lyp1Δ0 | This study |
| vi. | ||
| Y15584-4a | MATahis3Δ1 leu2Δ0 ura3Δ0 can1Δ0∷PGAL1-TADH1-PSTE2-his5+ lyp1Δ0 | This study |
| Y15591-5.2b | MATα his3Δ1 leu2Δ0 ura3Δ0 can1Δ0∷PGAL1-TADH1-PSTE2-his5+ lyp1Δ0 hmrΔ0∷URA3ca | This study |
| Y15591-15.3b | MATα his3Δ1 leu2Δ0 ura3Δ0 can1Δ0∷PGAL1-TADH1-PSTE2-his5+ lyp1Δ0 hmrΔ0∷URA3ca | This study |
| Y15591-21.4b | MATα his3Δ1 leu2Δ0 ura3Δ0 can1Δ0∷PGAL1-TADH1-PSTE2-his5+ lyp1Δ0 hmrΔ0∷URA3ca | This study |
| Y15583-13.2b | MATα his3Δ1 leu2Δ0 ura3Δ0 can1Δ0∷PGAL1-TADH1-PMFA1-his5+ lyp1Δ0 | This study |
| Y15586b | MATα his3Δ1 leu2Δ0 ura3Δ0 can1Δ0∷PGAL1-TADH1-PSTE3-his5+ lyp1Δ0 | This study |
| vii. | ||
| Y15580a | MATahis3Δ1 leu2Δ0 ura3Δ0 met17Δ0 hmlΔ0∷URA3ca | This study |
| Y15588-10.2a | MATahis3Δ1 leu2Δ0 ura3Δ0 can1Δ0∷PGAL1-TADH1-PSTE3-his5+ lyp1Δ0 hmlΔ0∷URA3ca | This study |
| Y15579-4a | MATahis3Δ1 leu2Δ0 ura3Δ0 can1Δ0∷PGAL1-TADH1-PMFα1-his5+lyp1Δ0 hmlΔ0∷URA3ca | This study |
| Diploids | ||
| Y15124a/b | MATa/MATα his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 LYS2/lys2Δ0 met17Δ0/MET17can1Δ0∷PGAL1-TADH1-PMFA1-HIS3/CAN1 | This study |
| Y15125a/b | MATa/MATα his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 LYS2/lys2Δ0 met17Δ0/MET17can1Δ0∷PGAL1-TADH1-PMFA1-LEU2/CAN1 | This study |
| Y15126a/b | MATa/MATα his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 lys2Δ0∷PGAL1-TADH1-PMFA1-HIS3/lys2Δ0 met17Δ0/MET17 | This study |
| Y15127a/b | MATa/MATα his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 met17Δ0/MET17 lys2Δ0∷PGAL1-TADH1-PMFA1-LEU2/lys2Δ0 | This study |
| Y15131a/b | MATa/MATα his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 MET17/met17Δ0 CAN1/can1Δ0∷PGAL1-ADH11term-PMFA1-HIS3 lys2Δ0∷PGAL1-TADH1-PMFA1-LEU2/LYS2 | This study |
| Y15132a/b | MATa/MATα his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 met17Δ0/MET17 CAN1/can1Δ0∷PGAL1-TADH1-PMFA1-LEU2 lys2Δ0∷PGAL1-TADH1-PMFA1-HIS3/LYS2 | This study |
| Y15572a/b | MATa/MATα his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 LYS2/lys2Δ0 met17Δ0/MET17can1Δ0∷PGAL1-TADH1-PMFA1-his5+/CAN1 HMR/hmrΔ0∷URA3ca | This study |
| Y15574a/b | MATa/MATα his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 LYS2/lys2Δ0 can1Δ0∷PGAL1-TADH1-PMFα1-his5+ | This study |
| Y15575a/b | MATa/MATα his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 met17Δ0/met17Δ0 LYP1/lyp1Δ0 CAN1/can1Δ0∷PMFA1-HIS3-PMFα1-LEU2 | This study |
| Y15576a/b | MATa/MATα his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 met17Δ0/MET17 lyp1Δ0/LYP1LYS2/lys2Δ0 CAN1/can1Δ0∷PGAL1-TADH1-PMFA1-his5+ HMR/hmrΔ0∷URA3ca | This study |
| Y15577a/b | MATa/MATα his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 lys2Δ0/LYS2 MET17/met17Δ0 can1Δ0∷PGAL1-TADH1-PMFα1-his5+/CAN1 lyp1Δ0/LYP1 | This study |
| Y15578a/b | MATa/MATα his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 MET17/met17Δ0 can1Δ0∷PGAL1-TADH1-PMFA1-his5+/can1Δ0∷PGAL1TADH1-PMFA1-his5+lyp1Δ0/lyp1Δ0 HMR/hmrΔ0∷URA3ca | This study |
| Y15583a/b | MATa/MATα his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 CAN1/can1Δ0∷PGAL1-TADH1-PMFA1-his5+HMR/hmrΔ0∷URA3ca LYP1/lyp1Δ0 | This study |
| Y15588a/b | MATa/MATα his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 met17Δ0/MET17 CAN1/can1Δ0∷PGAL1-TADH1-PSTE3-his5+hmlΔ0∷URA3ca/HML LYP1/lyp1Δ0 | This study |
| Y15591a/b | MATa/MATα his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 LYS2/lys2Δ0 can1Δ0∷PGAL1-TADH1-PSTE2-his5+/CAN1 HMR/hmrΔ0∷URA3ca lyp1Δ0/LYP1 | This study |
Roman numerals are referenced to supplemental Figure S1, supplemental Table S1, and materials and methods.