Figure 3.—

Characterization of scZorro3 retrotransposition events in S. cerevisiae. (A) New scZorro3 insertions after galactose induction. Top: Donor scZorro3 integrated at the LYS2 locus in an spt3Δ/rad52Δ strain (JHY339). H, HaeII. Blue bar indicates the position of the Zorro3 probe. Bottom: Southern analysis of galactose-induced scZorro3 HIS⁺ clones. Total genomic DNA was digested with HaeII and probed with indicated probes. (B) General structures of empty sites and insertions of cloned scZorro3 retrotransposition events (summarized in Table 1). Poly(A) tracts are highlighted in red and the subscript represents the average poly(A) length when multiple clones were sequenced. Deleted sequences are highlighted in orange.