TABLE 1.
NILs used to generate double introgression lines
| LA number | QTL status | Chromosome location | Introgression length (cM) | % genome |
|---|---|---|---|---|
| LA3975 | None | 3 | 12.1 | 0.0096 |
| LA3968 | None | 12 | 14.1 | 0.0112 |
| LA3964 | None | 10 | 22.5 | 0.0179 |
| LA3957 | None | 9 | 44.8 | 0.0356 |
| LA3947 | None | 6 | 8.6 | 0.0068 |
| LA3956 | Pollen | 9 | 57.4 | 0.0456 |
| LA3935 | Pollen | 4 | 53 | 0.0421 |
| LA3950 | Pollen | 7 | 33.8 | 0.0268 |
| LA3963 | Pollen | 10 | 30.3 | 0.0241 |
| LA3948 | Pollen | 7 | 50.4 | 0.0400 |
| LA3931 | Seed | 4 | 18.7 | 0.0149 |
| LA3939 | Seed | 5 | 25.8 | 0.0205 |
| LA3943 | Seed | 5 | 34 | 0.0270 |
| LA3915 | Seed | 1 | 34.8 | 0.0276 |
| LA3977 | Seed | 4 | 19 | 0.0151 |
LA number, seed accession identifier (see tgrc.ucdavis.edu). QTL status, five NILs contained pollen sterility QTL and five contained seed sterility QTL previously identified in the genomewide survey of hybrid incompatibility between these two species (Moyle and Graham 2005). The remaining five NILs had no detected effects on hybrid pollen and seed fertility but had introgression lengths (centimorgans) comparable to the sterility QTL NIL set. Note that segregation distortion detected here is unlikely to be systematically due to the direct effects of pollen or seed sterility QTL because: all known sterility QTL cause only partial sterility; lines carrying seed sterility QTL were preferentially used as pollen parents, and vice versa; our crossing methodology maximizes potential seed set (i.e., copious pollen is transferred and crosses are repeated multiple times to obtain sufficient seed); and, in only 4 of 31 cases where individual SH introgressions were significantly underrepresented in F2 populations (see text), was a parental line known to be partially sterile for the relevant parental function (i.e., seed sterility for maternal alleles, pollen sterility for paternal alleles).