TABLE 2.

Double introgression line F₂ families showing significant under- or overrepresentation of SH double homozygote genotypes

DIL family	NIL 1	NIL 2	χ2	P-value	Direction
36	LA3935	LA3956	5.109	0.024	Under
37	LA3950	LA3956	4.309	0.038	Under
44	LA3948	LA3950	7.175	0.007	Under
62	LA3935	LA3931	4.205	0.040	Over
69	LA3964	LA3939	4.051	0.044	Under
108	LA3964	LA3977	4.144	0.042	Under
110	LA3947	LA3977	4.275	0.039	Under
118	LA3939	LA3977	4.722	0.030	Over

Direction, under- or overrepresentation of double SH homozygotes. Under a χ² test with 1 d.f. we can detect a significant underrepresentation only when we find two or fewer double homozygotes in 120 genotyped individuals. Therefore, this test is relatively conservative with respect to detecting underrepresented double homozygotes. Supplemental Table 2 gives results for all pairwise and single tests of allelic and genotypic distortion.