Figure 3.—

The pds1Δ prophase arrest does not inhibit middle meiotic gene expression. (A) Wild-type (RSY335) and pds1Δ (RSY787) SK1/W303 cells were induced to enter meiosis at 23° and samples were taken at the times indicated (in hours). Total RNA was prepared and the transcript expression profiles of IME2, NDT80, SPS2, SPS4, and SPS100 were analyzed by Northern analysis. The approximate times of meiosis I (MI) and meiosis II (MII) as determined by DAPI analysis are indicated in the wild-type strain. ENO1 serves as a loading control. (B) Northern analysis of IME2, SPS2, and DIT1 mRNA expression in pds1Δ (RSY787) and pds1Δ spo13Δ (RSY795) SK1/W303 strains. (Bottom) Ethidium-bromide-stained rRNA is shown as a loading control.