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. 2009 Jan;181(1):65–79. doi: 10.1534/genetics.108.095513

Figure 6.—

Figure 6.—

Pds1p is required for normal SC formation. (A) Live-cell overlay images (GFP and Nomaski) of wild type (RSY335) and pds1Δ (RSY1433) strains expressing GFP-Zip1p 6 hr after transfer to SPM. The finger-like structure present in the wild-type cells represents individual chromosomes with formed SCs. (B) Fixed pds1Δ cells (RSY1433) harboring the GFP-ZIP1 expression plasmid 7.5 hr after transfer to SPM stained with DAPI (blue) examined under the fluorescent microscope. An expanded region indicated by the box is provided on the right (×600 magnification). (C) The cells described in B were subjected to indirect immunofluorescence staining for the nucleolar protein Nop1p. The images shown represent deconvoluted Z-stacks (0.6 μm slices) of Nomarski, GFP-Zip1p (green), Nop1p (red), and nuclei (blue). Magnification is ×1000.