TABLE 1.
Yeast strains used in this study
| Straina | Genotypeb | Background |
|---|---|---|
| RSY335a | MATa/MATα | SK1/W303 |
| RSY740a | MATa/MATα mad2∷TRP1 | SK1/W303 |
| RSY767a | MATa/MATα spo13∷TRP1 | SK1/W303 |
| RSY787a | MATa/MATα pds1∷URA3 | SK1/W303 |
| RSY795a | MATa/MATα pds1∷URA3 spo13∷TRP1 | SK1/W303 |
| RSY863a | MATa/MATα mad2∷TRP1 pds1∷URA3 rad17∷LEU2 | SK1/W303 |
| RSY864a | MATa/MATα mad2∷TRP1 pds1∷URA3 | SK1/W303 |
| RSY1343 | MATa/MATα ade1/ade2 cyh2/CYH2 his7-2/his7-1 leu1-12/leu1-c lys2-1/lys2-2 met13-d/met13-c trp5-d/trp5-c ura3/ura3∷LYS2 pds1∷URA3 | S288c |
| RSY1344 | MATa/MATα ade1/ade2 cyh2/CYH2 his7-2/his7-1 leu1-12/leu1-c lys2-1/lys2-2 met13-d/met13-c trp5-d/trp5-c ura3/ura3∷LYS2 | S288c |
| RSY1355a | MATa/MATα red1∷kanMX6 | SK1/W303 |
| RSY1356a | MATa/MATα mek1∷TRP1 | SK1/W303 |
| RSY1358a | MATa/MATα red1∷kanMX6 pds1∷URA3 | SK1/W303 |
| RSY1359a | MATa/MATα pds1∷URA3 mek1∷TRP1 | SK1/W303 |
| RSY1433a | MATa/MATα pds1∷ura3∷TRP1/pds1∷ura3∷LEU2 | SK1/W303 |
| RSY1453a | MATa/MATα ndt80∷LEU2/ndt80∷LEU2 pds1∷ura3∷TRP1/pds1∷URA3 | SK1/W303 |
| RSY1456a | MATa/MATα ndt80∷LEU2/ndt80∷LEU2 | SK1/W303 |
| RSY1536a | MATa/MATα pch2∷TRP1/pch2∷TRP1 | SK1/W303 |
| RSY1537a | MATa/MATα pds1∷ura3∷LEU2/pds1∷URA3 pch2∷TRP1/pch2∷TRP1 | SK1/W303 |
| KCY198a | MATa/MATα spo11∷kanMX6 | SK1/W303 |
| KCY207a | MATa/MATα spo11∷kanMX6 pds1∷URA3 | SK1/W303 |
| KCY257 | MATa/MATα his4/HIS4 leu2 trp1/TRP1 ura3 arg4(EV)/arg4(Bgl) | SK1 |
| KCY263a | MATa/MATα bub2∷TRP1 | SK1/W303 |
| KCY269a | MATa/MATα bub2∷TRP1 pds1∷URA3 | SK1/W303 |
| KCY274 | MATa/MATα his4/HIS4 leu2 trp1/TRP1 ura3 arg4(EV)/arg4(Bgl) pds1∷URA3 | SK1 |
| KCY345 | MATa/MATα leu2-3,112/LEU2 his3Δ200/HIS3 ura3-52 gal1/GAL1 | A364A |
| KCY348 | MATa/MATα leu2-3,112/LEU2 his3Δ200/HIS3 ura3-52 gal1/GAL1 pds1-1 | A364A |
| KCY385a | MATa/MATα rec8∷TRP1 | SK1/W303 |
| KCY392a | MATa/MATα REC8-3HA∷TRP1 | SK1/W303 |
| KCY398a | MATa/MATα rec8∷TRP1 spo11∷kanMX6 | SK1/W303 |
| KCY399a | MATa/MATα rec8∷TRP1 spo13∷TRP1 | SK1/W303 |
| KCY427 | MATa/MATα ura3 trp1 leu2 rad50S∷LEU2 | SK1 |
| KCY428 | MATa/MATα ura3 trp1 leu2 arg4(Nsp)/arg4(Bgl) | SK1 |
| KCY429 | MATa/MATα ura3 trp1 leu2 rad50S∷LEU2 pds1∷URA3 | SK1 |
| KCY430 | MATa/MATα ura3 trp1 leu2 arg4(Nsp)/arg4(Bgl) pds1∷URA3 | SK1 |
| KCY444a | MATa/MATα rad17∷LEU2 | SK1/W303 |
| KCY445a | MATa/MATα rad17∷LEU2 mad2∷TRP1 | SK1/W303 |
| KCY447a | MATa/MATα ubr1∷kanMX6 REC8-3HA∷TRP1 | SK1/W303 |
| KCY448a | MATa/MATα ubr1∷kanMX6 REC8-3HA∷TRP1 pds1∷URA3 | SK1/W303 |
| KCY392a | MATa/MATα REC8-3HA∷TRP1 | SK1/W303 |
| KCY472a | MATa/MATα REC8-3HA∷TRP1 pds1∷URA3 | SK1/W303 |
| KCY450a | MATa/MATα rad9∷LEU2 rad17∷LEU2 | SK1/W303 |
| KCY453a | MATa/MATα rad9∷LEU2 rad17∷LEU2 pds1∷URA3 | SK1/W303 |
a
Strain also harboring cyh2r-z ho∷LYS2 leu2∷hisG lys2 trp1∷hisG ura3 alleles.
b
All alleles listed are homozygous unless indicated otherwise. All strains were derived in this study except RSY335 (Cooper et al. 1997).