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. 1996 Dec 10;93(25):14776–14781. doi: 10.1073/pnas.93.25.14776

Figure 4.

Figure 4

Analysis of transgenic skin protein extracts by SDS/PAGE and immunoblotting. (Upper) Mouse K6 (mK6) immunoblot. (Lower) Myc tag immunoblot. Lanes: 1–3, 15 μg protein loaded; 4–7, 5 μg protein loaded. Samples are as follows: lane 1, control mouse, intact skin; lane 2, 19–1p mouse (2.5-kb promoter), intact skin; lane 3, 21–1p mouse (5.2-kb promoter), intact skin; lane 4, 19–2m mouse (2.5-kb promoter), PMA-treated skin; lane 5, 19–1p mouse (2.5-kb promoter), PMA-treated skin; lane 6, 21–1p mouse (5.2-kb promoter), PMA-treated skin; lane 7, 21–1m transgenic mouse (5.2-kb promoter), PMA-treated skin; lane 8 (myc immunoblot only); 20 ng of purified recombinant ΔK6a-myc protein. Note the strong induction in K6 protein after PMA treatment (lanes 4–7; mK6 blot), and the variable extent of ΔK6a-myc product among various transgenic lines (myc blot).