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Immunodetection and localization of GldH. (A) Cell extracts were examined for GldH by Western blot analysis. Lane 1, wild-type F. johnsoniae. Lane 2, CJ1043 (Tn4351 insertion in gldH). Lane 3, CJ1043 with pMM293, which carries gldH. (B) Cell fractions were examined for GldH by Western blot analysis. Lane 1, soluble fraction. Lane 2, membrane fraction.
