Table 4.

PCR primer pairs used for genomic cloning of the different peach/almond allergen genes and for (bin) mapping the genes

Primers group	Reference Sequence 1	Primer name	Primer sequence (5' – 3')	Tm °C/cycles		Product	Gene
				Pfu	Taq	(nt)
1	DQ251187	PpPr10f PpPr10r	ACCATGGGTGTCTTCACATA AATTTAGTTGTAGGCATCGG	56/30	58/2	586/623	Pru p/du 1.01/05
2	DY646736	Pp1-2f Pp1-2r	GTTTGTCCTTTAAACTCTCC GATTTAGTTGTAGGCATCGG	53/30	55/2	762/765	Pru p/du 1.02
3	DY647300	Pp1-3f Pp1-3r	CTTTGATCAGTTTCCCAAGT TTAGTTGTAGGCATCAGGGT	53/30	55/2	624	Pru p/du 1.03
4	DY653062	Pp1-4f1 Pp1-4r	TTTACAGAATCATGGGTGTG TTAGTTGTAGGCATCATGGT	53/30	55/2	615/622	Pru p/du 1.04
5	GW1	Pp1-1A-GSP1 Pp1-1A-GSP2	AACCTTCACCAAAGCTAGTCTTCTTGATG GTCAGCATCCAGAACAAGGGCTTTGAACA			1100	Pru p/du 1.05 /6A/C
6	GW2	P1-1A3-GSP1 P1-1A3-GSP2	GGTGTCTTCACATACTCAGACGAGTC CCACCTCAGTCATCCCCCCACCAAGA			1546	Pru p/du 1.06A/C
7		P1-1-6 PpPr10r	CACCTCCAGTGCTCATAGC See above	57/30	59/2	720	Pru p/du 1.05
8		P1-7f P1-7r	ATGGGTGTCTTCACATACTCAG TTTAGTTGTAGGCATCTGGGT	57/30	59/2	593	Pru p/du 1.06A/B
9		P1-8f P1-8r2	TAGTTATGAGTTGCTTGCAATGCT GGGCATGCTTGCTTTGGTAACTC	58/30	60/2	1346	Pru p/du 1.06C
10		P1-8f P1-9r	See above GAAAGTTCCAAAGTACATGTGC	58/30	60/2	1908	Pru p/du 1.06A
11	AF362988	PpTL2f PpTL2r	ACAGCAAGCCAATTAAGACA GCTTATGGGCAGAATGTGATGAG	56/30	58/2	879/892	Pru p/du 2.01A/B
12	AF362987	PpTL1f PpTL1r	ATGATGAAGACCCTAGGAGCAG GTCGAGAGTCCTATCTTTAT	56/30	58/2	974	Pru p/du 2.02
13	GW3	PTLP1-GSP1 PTLP1-GSP2	GATAATTGAGGATTTCCAAAGGAGGCTG AGGAGGGTTAAGCTGAGGCTGAGGACTG			500/1500	Pru p/du 2.02
14	BF717226, DW349103	PpTLP3f PpTLP3r	AAGTAAAGTTCTTTGGCGTC CACGTGGCACCACAAGCATC	53/30	55/2	659	Pru p/du 2.03
15	GW4	PruTLP3-GSP1 PruTLP3-GSP2	AGTACTCACCCACCAACTTCTCCTGCGT GCTACTTTAGCCGAGTTCACTCTCAACG			800/1000	Pru p/du 2.03
16	Consensus of 29 ESTs	PpTLP4f PpTLP4r	ACTTGTCTGAACTTATGGCTCC GAAGTTAGAAGAAGAGCTGCG	58/30	60/2	1800/1825	Pru p/du 2.04
17	AY620230 X96714	PpLTP1f1 PpLTP1r	ATCATAGTCAAGAGAGATGG CCCTAAGTGGATCACATAGC	52/30	54/2	645	Pru p/du 3.01
18	AY093699 X96716	PpLTP2f PpLTP2r	TATCAGCTTTACTTACGACG CTGGCTTCCACAGAAACCTC	58/30	60/2	513/514	Pru p/du 3.02
19	X96715	PpLTP3f PpLTP3r	CCCAAGCGAAAGAAACACTA CATCTCATATCATCCTTCCA	58/30	60/2	527/528	Pru p/du 3.03
20	AJ491881 AY081852	Pp4.01f Pp4.01r	AGAAGAAATCAGAAGCAACG AAATAGTCACTCGGAGCAAT	51/30	53/2	1041	Pru p/du 4.01
21	AJ491882	Pp4.02f Pp4.02r1	CAGCAACAACAACAAAGATG TCTAGAGACCCTGCTCAACC	53/30	55/2	754	Pru p/du 4.02

¹GW-genome walking, only gene specific primers (GSP) shown, according to sequences obtained with previous primer group, representative reference EST accessions for group 16: [GenBank:DY634569; DY644567; DY651848; DY634415]