Table 1.
Summary of EST, BLAST, and SignalP analyses from H. erato and H. melpomene male accessory gland cDNA libraries.
| Heliconius erato | Heliconius melpomene | |
| EST results | ||
| Number of clones sequenced | 1152 | 1148 |
| High Quality ESTs1 | 936 (81%) | 1033 (89%) |
| Number of unigenes | 371 | 340 |
| Average sequence length (base pairs) | 641 | 597 |
| BLAST results2 | ||
| Unigenes with significant BLAST hits to GenBank protein or nucleotides3 (E-value < 10-5) | 257 (69%) | 235 (69%) |
| Unigenes with significant BLASTX hits to GenBank proteins (E-value < 10-5) | 216 (58%) | 218 (64%) |
| Unigenes with significant BLASTN hits to GenBank nucleotides (E-value < 10-5) | 151 (40%) | 150 (44%) |
| SignalP results2 | ||
| Unigenes with predicted signal peptides4 | 86 (24%) | 92 (28%) |
1ESTs > 100 bp after trimming raw sequences of vector sequence, low-scoring base calls, and poly-A tails. 2Percentages given in reference to total number of unigenes. 3Includes results from both BLASTN and BLASTX searches. 4We required a positive result from both hidden markov models and neural network methods implemented in SignalP.