Table 1.
Designations and sequences of oligonucleotides
| Name | Sequence (5'-3') | GHRLOS Exon | Ta (°C) | PCR Cycles |
| 5'2-out-F | GATGGCGATGAATGAACACTG | N/A | ||
| 5'2-out-R | AATCATCTCAGGAATACCTGGA | 2 | 60 | 35 |
| 5'2-in-F | ATGAATGAACACTGCGTTTGC | N/A | ||
| 5'2-in-R | AAATGGAAGAGATGAGGCGC | 2 | 61 | 35 |
| Ex1-cRNA-F | CATACAGTTTGAACATTTATTCGCCTCC | 1 | ||
| Ex1-cRNA-R | CTAATACGACTCACTATAGGGAGACTCTCTCTAAGTTTAGAAGCGCTCATCTG | 1 | 62 | 25 |
| 3'2OF | GAGAGCGCCTCATCTCTTCC | 2 | ||
| 3'2OR | GCGAGCACAGAATTAATACGACTC | N/A | 63 | 35 |
| 3'2IF | ATGATTTATTGGAGCTCAAAGC | 2 | ||
| 3'2IR | GAATTAATACGACTCACTATAGGT | N/A | 57 | 15 |
| 3'4 | TACGGAACAGAGGAGAGATGC | 4 | 60 | 35 |
| 3' -RACE-adapter | GCGAGCACAGAATTAATACGACTCACTATAGGTTTTTTTTTTTTVN | N/A | N/A | |
| IPCR-out-F | AAATCCCACCTTTAGTCCCA | 4 | 60 | 35 |
| IPCR-in-F | CTGCCACCTGAGTGTAGAC | 4 | 60 | 20 |
| IPCR-ALL-R | CACAGGCTTGGAGACTTCC | I | ||
| Pthioate-hex | NNNNsNsN | N/A | 30 | N/A |
| Phospho-dT | P- GGCCACGCGTCGACTAGTAC(T)18 | N/A | 55 | N/A |
| GHRL-Real-RT-LK | CGACTGGAGCACGAGGACACTGAGCCAGAGAGCGCTTCTAAACTTA | N/A | N/A | |
| GHRL-Real-F | GCCCCAGCCGACAAGTG | N/A | 60 | 40 |
| Ito4-F | CATGGAAGTCTCCAAGCCTG | I | ||
| Ito4-R | CTGCTCTACTGCCTCAATGTC | 4 | 63 | 34 |
| GHRLOS-Real-RT-LK | CGACTGGAGCACGAGGACACTGACAATCCTCCCTGAGGTTGATCT | 4 | N/A | |
| GHRLOS-Real-F | CATTGAGGCAGTAGAGCAGTTGA | 4 | ||
| LK | CGACTGGAGCACGAGGACACTGA | N/A | 60 | 40 |
| Ex4-TaqMan | FAM-TGCCGAATGACCACCTACCCTGACTT- BHQ1 | 4 | ||
| 18S-Real-F | TTCGGAACTGAGGCCATGAT | N/A | ||
| 18S-Real-R | CGAACCTCCGACTTTCGTTCT | N/A | 60 | 40 |
| F_CAGE | GGGACTGCCTGTAATAGCAC | I | ||
| R_CAGEout | CACGACTGTTGTACAAGCTC | 1 | 60 | 35 |
| R_CAGEin | GGAGGCGAATAAATGTTCAAACTG | 1 | 61 | 30 |
| ChiOut-F | TGAAAGCCCAGAAGGAGGA | N/A | ||
| ChiOut-R | TCTAAGTTTAGAAGCGCTCATCTG | 1 | 63 | 35 |
| ChiIn-F | CAGAAGGAGGACGATGTGG | N/A | ||
| ChiIn-R | CACGACTGTTGTACAAGCTC | 1 | 62 | 30 |
| UIS231-F | ACAAGTTCAACGATGTGGTG | N/A | ||
| UIS231-R | CAAGTGTGAATAATAACCAAGCCC | N/A | 55 | 40 |
T7 promoter sequence in PCR primers is underlined. Linker sequence (LK) in the primer GHRLOS-Real-RT-LK and GHRL-Real-RT-LK is shown in bold. In primer 3' -RACE-adapter V denotes an A/G/C residue and N denotes A/G/C. Lowercase letters in primer Pthioate-hex denotes phosphothioate linkages. An uppercase P in primer Phospho-dT indicates that the synthetic oligonucleotide was 5' phosphorylated. Annealing temperatures (Ta) of oligonucleotides employed in PCR is shown. The GHRL or GHRLOS exon location of nucleotides are listed, while oligonucleotides spanning synthetic sequences (adapters and linkers) or genes other than GHRL and GHRLOS are denoted as N/A.