TABLE 2.
Summary of the characterized phytoplasmal (AY) clonesa
Clone information | Blast results
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Clone | GenBank accession no. | % GC | ORF no. | Base position and orientationb | Homologous protein | Organism | % Identity | % Similarity |
A1 | AY191277 | 54 | 1 | 89-988 + | Hypothetical proteinc | |||
2 | 153-1067 + | Hypothetical protein | ||||||
3 | 982-350 − | Hypothetical protein | ||||||
A2 | AY191278 | 36 | 1 | 605-315 − | Conserved hypothetical protein | Helicobacter pylori 26695 | 26 | 47 |
2 | 648-818 + | Hypothetical protein | ||||||
3 | 1309-1127 − | Hypothetical protein | ||||||
4 | 1592-1443 − | gtf2ird2 | Mus musculus | 40 | 59 | |||
5 | 1640-1822 + | Hypothetical protein | Plasmodium falciparum 3D7 | 46 | 64 | |||
6 | 2248-1901 − | IpaD | Shigella sonnei | 31 | 58 | |||
7 | 2428-2261 − | Hypothetical protein | ||||||
8 | 2460-2636 + | Similar to hornerin | Mus musculus | 40 | 52 | |||
9 | 2850-3200 + | Putative dipeptidase protein | Sinorhizobium meliloti | 50 | 56 | |||
10 | 3172-2996 − | Hypothetical protein | ||||||
11 | 3107-3370 + | Hypothetical protein | ||||||
12 | 3497-3823 + | agCP3554 | Anopheles gambiae | 51 | 69 | |||
A10 | AY191279 | 24 | 1 | 2-238 + | rRNA methylase | Thermoanaerobacter tengcongensis | 40 | 60 |
2 | 1502-240 − | Threonine dehydratase | Bacillus halodurans | 56 | 75 | |||
A93 | AY191280 | 25 | 1 | 214-1 − | NADH dehydrogenase 1 | Mitochondrion of Flexarida chaotica | 79 | 91 |
A115 | AY191281 | 23 | 1 | 268-1 − | Hypothetical protein | |||
2 | 69-350 + | EF-G | Porphyromonas gingivalis | 58 | 83 | |||
3 | 326-628 + | rpS10 | Leptospira interrogans | 59 | 79 | |||
4 | 908-661 − | Hypothetical protein | ||||||
5 | 665-909 + | rpL3 | Thermoanaerobacter tengcongensis | 59 | 78 | |||
A167 | AY191282 | 38 | 1 | 725-1 − | DNA polymerase | Mitochondrion of Neurospora intermedia | 32 | 48 |
2 | 774-451 − | Putative DNA polymerase | Mitochondrion of Porphyra sp. | 30 | 55 | |||
A182 | AY191283 | 26 | 1 | 56-400 + | rpL18 | Bacillus stremophilus | 45 | 57 |
2 | 413-934 + | rpS5 | Shewanella oneidensis MR-1 | 46 | 64 | |||
3 | 939-1105 + | rpL15 | Neisseria meningitidis MC58 | 76 | 85 | |||
A226 | AY191284 | 29 | 1 | 230-1 − | Cytochrome c oxidase polypeptide III | Mitochondrion of Spodoptera frugiperda | 67 | 77 |
A239 | AY191285 | 24 | 1 | 8-361 + | Acetohydroxy acid synthase II | Escherichia coli O157:H7 EDL933 | 35 | 48 |
2 | 349-609 + | Hypothetical protein | ||||||
3 | 600-1634 + | Ketol-acid reductoisomerase, mito- hondrial precursor | Neurospora crassa | 49 | 66 | |||
A244 | AY191286 | 27 | 1 | 2-871 + | 2-Isopropylmalate synthase | Aquifex aeolicus | 53 | 71 |
2 | 872-1885 + | 3-Isopropylmalate dehydratase large subunit | Oceanobacillus iheyensis | 49 | 68 | |||
A276 | AY191287 | 34 | 1 | 78-164 + | Surface protein, putative | Shewanella oneidensis MR-1 | 39 | 58 |
A290 | AY191288 | 18 | 1 | 95-493 + | Signal peptidase 1 | Chlorobium tepidume TLS | 38 | 57 |
2 | 450-647 + | rpS21 | Myxococcus xanthus | 44 | 68 | |||
3 | 651-963 + | Shikimate 5-dehydrogenase | Clostridium perfringens | 36 | 63 | |||
A293 | AY217338 | 33 | 1 | 617-378 − | Hypothetical protein | |||
2 | 629-883 + | Hypothetical protein | ||||||
3 | 1214-702 − | RNA polymerase I | Plasmodium falciparum 3D7 | 44 | 67 | |||
A294 | AY191289 | 30 | 1 | 133-2 − | Putative transport protein | Escherichia coli | 39 | 51 |
A295 | AY1912290 | 34 | 1 | 188-1 − | Hypothetical protein | Leptospira interrogans serovar Lai strain 56601 | 29 | 52 |
2 | 13-210 + | Putative N-acetyltransferase Camello 4 | Mus musculus | 31 | 54 | |||
A314 | AY191291 | 25 | 1 | 5-981+ | NADH dehydrogenase subunit 5 | Mitochondrion of Pyrocoelia rufa | 51 | 66 |
A315 | AY191292 | 48 | 1 | 128-2 − | Integrase protein | Salmonella enterica serovar Typhimurium | 54 | 62 |
A328 | AY191293 | 30 | 1 | 106-1197 + | Hypothetical protein | |||
A352 | AY217339 | 33 | 1 | 1314-1084 − | Caspase-9 long chain | Mouse | 50 | 62 |
2 | 1231-1551 + | Outer capsid protein | Porcine group C rotavirus | 40 | 65 | |||
3 | 1343-1510 + | Hypothetical protein | ||||||
4 | 1886-2071 + | Hypothetical protein | ||||||
5 | 2664-2825 + | Hypothetical protein | ||||||
3 | 508-359 − | Hypothetical protein | /PICK> | |||||
A370 | AY217340 | 40 | 1 | 534-1 − | Reverse transcriptase | Picea glauca | 49 | 69 |
2 | 1250-375 − | Putative Gag-Pol polyprotein | Oryza sativa | 37 | 56 | |||
3 | 1639-1328 − | Putative copia-type polyprotein | Oryza sativa | 51 | 64 | |||
A380 | AY191294 | 23 | 1 | 2-172 + | Cytochrome oxidase subunit III | Mitochondrion of Manis tetradactyla | 45 | 60 |
2 | 225-599 (+) | NADH dehydrogenase subunit 3 | Anopheles gambiae | 64 | 85 | |||
3 | 1302-709(−) | Cyclophilin D | Dictyostelium discoideum | 40 | 56 | |||
A424 | AY191295 | 29 | 1 | 309-476 + | Hypothetical protein | |||
2 | 322-471 + | Hypothetical protein | ||||||
A427 | AY191296 | 22 | 1 | 2-160 + | Threonyl-tRNA synthetase | Buchnera aphidicola strain sg | 92 | 94 |
A440 | AY191297 | 29 | 1 | 4-429 + | Elongation factor Tu | Cellulophaga lytica | 76 | 88 |
2 | 652-891 + | rpL11 | Chlamydia muridarum | 63 | 86 | |||
A448 | AY191298 | 22 | 1 | 194-1 − | rpS8 | Listeria innocuus | 62 | 81 |
2 | 200-723 + | rpL6 | Oceanobacillus iheyensis | 43 | 60 | |||
A449 | AY191299 | 31 | 1 | 743-1012 + | Hypothetical protein | |||
A450 | AY191300 | 39 | 1 | 175-2(−) | Conserved hypothetical protein | Agrobacterium tumefaciens | 33 | 53 |
2 | 215-18 − | Nef attachable protein | Homo sapiens | 38 | 58 | |||
A458 | AY191301 | 45 | 1 | 222-1 − | Yjcl protein | Bacillus halodurans | 33 | 52 |
A463 | AY191302 | 32 | 1 | 357-37 − | DNA-adenine methyltransferase | Actinobacillus actinomycetemcomitans | 28 | 46 |
2 | 269-436 + | Hypothetical protein | ||||||
3 | 542-282 − | Hypothetical protein | ||||||
4 | 530-781 + | Retroelement | Tricholoma matsutake | 27 | 44 | |||
A526 | AY217341 | 29 | 1 | 129-28 − | Fusolin | Nucleopolyhedrovirus of Autographia californica | 48 | 68 |
A533 | AY217342 | 34 | 1 | 655-825 + | Hypothetical protein | |||
2 | 855-1061− | ATPase subunit 6 | Kinetoplast of Trypanosoma brucei | 31 | 45 | |||
A540 | AY217343 | 41 | 1 | 1-256 + | agCP1945 | Anopheles gambiae | 34 | 49 |
Sequences of over 50 amino acids lacking an initial methionine or a stop codon (usually ORFs at the ends of the clones) are also included.
The orientation from the T7 promoter end of the clone is arbitrarily designated +.
A sequence of at least 50 amino acids with no match by BlastX is termed a hypothetical protein.